It is suitable for in vitro qualitative detection of sperm SP10 protein in human semen. The WHO "Human Semen Test and Processing Laboratory Manual" (Fifth Edition) mentions that the main factors affecting male infertility are: semen volume, total number of sperm, sperm concentration, liquefaction time, semen pH value, sperm morphological rate, etc. , And sperm concentration detection is an important indicator of male infertility.

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Product Description

[Sample]

[Intended use]

This product is used for the qualitative detection of sperm SP10 protein in human semen in vitro.WHO laboratory manual for the examination and processing of human semen, th ed. mentioned that the main factors affecting male infertility are: semen volume, total number of sperm, sperm concentration.liquefaction time, semen pH value, normal sperm morphology rate, etc, where sperm concentration is an important indicator to measure male infertility. In this manual, the normal minimum value of sperm concentration is 15x106/mL, and when the sperm concentration is less than 15x106/mL, it is considered that there is dificulty in fertility. However, male infertility needs to be combined with other indicators to make a final judgment.Based on several anatomical, genetic, and biochemical features, the SP10 protein.which is only expressed in the testis and is a differentiation marker for the final step of the spermatogenesis process, is validated as an analyte for sperm concentration detection. According to the research data.there is a certain linear relationship between the concentration of SP10 protein and the concentration of sperm, that is, when the concentration of sperm is more than 15x106/mL, the detection of SP10 protein is positive. Therefore, sperm concentration can be used in the auxiliary diagnosis and curative effect observation of male infertility, and provide guidance for the reproductive planning of age-appropriate couples. This product can be used not only for the preliminary screening of sperm concentration in medical institutions. but also for consumers to test at home to preliminarily judge whether the sperm concentration level meets the standard.

The colloidal gold method is currently the most commmonly used detection method for sperm SP10 protein in clinic or laboratory.

[Testing principle]

The product adopts the principle of double antibody sandwich. When the concentration of SP10 protein in the semen sample is equal to or higher than the minimum detection limit, the sperm SPI0 protein in the semen reacts with the colloidal gold-labeled mouse anti-human sperm SP10 protein monoclonal antibody (SP10 mAb1) on the binding pad to form a labeled antibody-antigen complex, the complex is chromatographed upward by capillary action, captured by the detection line T-line mouse anti-human sperm SP10 protein monoclonal antibody (SP10 mAb2) coated on a nitrocellulose membrane, and a red band appears. The complex continues to be chromatographically upward, and is captured by the quality control line (C line) antibody (goat anti-mouse IgG polyclonal antibody) coated on the nitrocellulose membrane, and a red band appears. When there is no sperm SP10 protein or the concentration of spermSP10 protein is lower than the minimum detection limit, the detection line (T line) will not show color.Regardless of the concentration of sperm in the sample to be tested, the quality control line (C line) will form a red color band visible to the naked eye, which is the standard for judging whether the sample volume is sufficient and whether the chromatography process is normal, and also serves as the internal control of this reagent.

Key Advantages

• High Accuracy.
• Easy to Use.
• Fast Detection: Result in 5 minutes.
• No equipment needed.
• Multiple Packaging Specifications: 1 Test/Box or 25 Tests/Box or 50 Tests/Box.

Operation Flow & Result Interpretation